Journal: Shock

Article Title: Mass Spectometry-Based Protein Patterns in the Diagnosis of Sepsis/Systemic Inflammatory Response Syndrome

doi: 10.1097/shk.0b013e318237ea7c

Figure Lengend Snippet: FIG. 3. Representative examples of normalized ProteinChip array profiles of SIRS-PCT+ and sepsis samples. Representative SELDI-TOF-MS protein pattern from patients of the different investigated groups as illustrated in gel view. Left: Chip type Q10; low range, 4.4 to 5 kd Chip type Q10; peak cluster C1_4.7. Middle: Chip type Q10; middle range, 12.5 to 15 kd; peak cluster C1_13.8. Right: Chip type Q10; high range, 30 to 60 kd; peak cluster QC_55.7. Peak clusters differentiating SIRS-PCT+ and sepsis patients are highlighted by arrows and boxes.

Article Snippet: For protein chip analysis, 40-2L samples were denatured with 60 2L denaturing buffer (9 M urea, 1% CHAPS) and incubated for 30 min; 20 2L of the denatured sample was diluted 1:5 in 0.1 mol/L Tris-HCl pH 9.0 (Q10) or 0.05 mol/L HEPES pH 7.3 (CM10) and loaded on Q10 ProteinChip arrays (strong anion exchange array; Bio-Rad, Hercules, Calif) and CM10 ProteinChip arrays (weak cation exchange array; Bio-Rad, Hercules, Calif), respectively, after appropriate chip pretreatment and washing.

Techniques: